Ultrasonic cell crusher old XO series

Product Description

Ultrasonic cell disruptor (ultrasonic disruptor) is a multifunctional and multi-purpose instrument that uses strong ultrasound to generate cavitation effect in liquid for ultrasonic treatment of substances. It can be used for the fragmentation of various animal and plant cells, virus cells, as well as for emulsification, separation, homogenization, extraction, defoaming, cleaning, and accelerating chemical reactions. Widely used in fields such as biochemistry, microbiology, medicinal chemistry, surface chemistry, physics, zoology, etc.

Main technical parameters:

Working principle and application of ultrasonic cell disruptor:

The effects of ultrasound on cells mainly include thermal effects, cavitation effects, and mechanical effects. The thermal effect is that when ultrasound propagates in a medium, frictional forces hinder the molecular vibrations caused by ultrasound, converting some of the energy into local high heat (42-43 ℃). This is because the critical lethal temperature of normal tissue is 45.7 ℃, and tumor tissue is more sensitive than normal tissue, resulting in metabolic disorders of tumor cells at this temperature, DNA、RNA、 Protein synthesis is affected, resulting in the killing of cancer cells while normal tissues are not affected. The cavitation effect is the formation of air bubbles in living organisms under ultrasound irradiation. With the vibration and violent explosion of the bubbles, mechanical shear pressure and turbulence are generated, causing tumor bleeding, tissue disintegration, and even necrosis. In addition, the rupture of cavitation bubbles generates instantaneous high temperature (about 5000 ℃) and high pressure (up to 500 × 104Pa), which can cause the thermal dissociation of water vapor OH radicals and The H atom is composed of OH radicals and The oxidation-reduction reaction caused by H atoms can lead to polymer degradation, enzyme inactivation, lipid peroxidation, and cell killing. Mechanical effect is the primary effect of ultrasound. During the propagation of ultrasound, the alternating compression and extension of medium particles create pressure changes, causing damage to cell structure. The strength of the killing effect is closely related to the frequency and intensity of ultrasound.
The principle of an ultrasonic cell disruptor is to convert electrical energy into sound energy through a transducer. This energy is then transformed into dense small bubbles through a liquid medium (such as water), which quickly explode and generate enormous energy, thereby breaking down cells and other substances (commonly known as the "cavitation effect"). Ultrasonic cell disruptor is a multifunctional and multi-purpose instrument that generates cavitation effect in liquid; It can be used for the fragmentation of various animal and plant cells, virus cells, bacteria, and tissues, as well as for the fragmentation and recombination of various inorganic substances. It can also be used for emulsification, separation, homogenization, extraction, defoaming cleaning, and accelerating chemical reactions.

Ultrasonic cell disruptors have a wide range of applications, such as:
1. Ultrasonic extraction of biological nanoparticles (ultrasonic chemical synthesis method)
In ultrasonic chemical reactions, the cavitation effect of sound waves plays a key role. During the irradiation process of ultrasonic waves, cavitation bubbles will form, grow, and collapse in the liquid. When the cavitation bubbles collapse, a strong pressure pulse covering them will be generated, producing many unique properties, such as high temperatures of up to 5000K and pressures greater than 200Mpa. This is the energy source of ultrasonic chemical synthesis, which can be used to synthesize nanoparticles on the surface of some special powders.
2. Ultrasonic pharmaceuticals
(1) Dispersion of injectable pharmaceutical substances - mixing phospholipids and cholesterol with drugs in aqueous solution using appropriate methods, and dispersing them by ultrasound to obtain smaller particles for intravenous injection.
(2) Herbal extraction - using ultrasound to disperse and destroy plant tissues, accelerating solvent penetration through tissues, and improving the extraction rate of effective ingredients in Chinese herbal medicine. It takes more than 5 hours for all alkaloids in the bark of Jinjina tree to be invaded by general methods, and it only takes half an hour to complete by ultrasonic dispersion.
(3) Preparation of suspension - Under ultrasonic cavitation and strong stirring, a solid drug is dispersed in an aqueous solution containing surfactants, which can form an oral or intravenous suspension of about 1um. Examples include "intravenous camptothecin suspension", "liver contrast agent", and "barium sulfate suspension".
(4) Preparation of vaccines - cells or bacteria are dispersed and killed by ultrasound, and then vaccines are made using appropriate methods.
3. Ultrasonic dispersion of cosmetics
In order to further extract the essence of drugs and refine the particles, save production costs, achieve dispersion and emulsification effects, make cosmetics penetrate deeper into the inner layer of the skin, allow the skin to absorb well, and play the efficacy and role of drugs, ultrasonic emulsification can achieve extraordinary results. By using ultrasonic dispersion, it is not necessary to use emulsifiers to disperse the fine particles of wax and paraffin emulsion, toner and other oils. The dispersed particles of paraffin in water can have a diameter of less than 1um.
4. Ultrasonic assisted aging technology for wine
A bottle of fine wine is favored for its mellow taste, soft and mellow texture, and rich aroma. People often use aged wine to describe the preciousness of wine. A bottle of aged wine from the last century is priced at tens of thousands of yuan, and the meaning of its price lies in the storage time. The main controlling factors of alcohol are chemical changes, namely the formation of acids, which are further esterified, and esters participate in the association of ethanol and water. Newly produced wine contains alcohol and has a spicy and pungent taste, which takes a long time to dissipate. This slow change is called wine aging. Using ultrasonic treatment with a power of 1.6KW and a frequency of 17.5-22KHZ for 5-10 minutes can shorten the aging time of wine by 1/3 to 1/2.
5. Ultrasonic treatment of silicon scale
Therefore, ultrasonic cell disruptors can and have been widely used in teaching, research, and production in fields such as biochemistry, microbiology pharmacology, physics, zoology, agriculture, medicine, and pharmaceuticals.
In the production of waste paper deinked pulp, in order to ensure the ultimate quality of the pulp and paper, as well as the papermaking requirements of the paper machine, strict requirements are placed on the removal rate of adhesives and the residual rate of ink particles. Therefore, most deinked production lines use thermal dispersion equipment to decompose larger adhesives into small particles that are not visible to the naked eye and completely remove ink particles that have not completely detached from the fibers, in order to ensure the quality of the pulp. To improve the quality of pulp, especially whiteness, most waste paper production lines add chemicals such as hydrogen peroxide (bleaching agent), sodium hydroxide, sodium silicate (stabilizer), EDTA, etc. before thermal dispersion, in order to achieve high-temperature and high concentration bleaching through the mixing effect of thermal dispersion.
Analysis of the Causes of Silicon Scale Formation
Simply put, the formation of scale layer is caused by sodium silicate and calcium and magnesium ions in water. The main reasons are as follows:
(1) Sodium silicate is a strong base and weak acid salt, and its solution is alkaline. At a high thermal dispersion temperature (around 90 ℃), the following hydrolysis reaction occurs:
SiO3- + H2O = HSiO3- + OH-
HSiO3-+ H2O =H2SiO3 + OH-
After hydrolysis of sodium silicate, a stable silica sol is generated, which rapidly condenses and gradually forms a jelly like gel like substance. In the case of a certain gap between the gears, the slurry can reach the area with low friction, which will become a place where this gel like substance and excess sodium silicate deposit, gradually forming a scale layer.
(2) In hydrogen peroxide bleaching, when calcium and magnesium ions are present simultaneously, sodium silicate preferentially reacts with magnesium ions to form magnesium silicate precipitate. This precipitate is flexible, talc like, and does not adhere to the surface of stainless steel, but calcium silicate precipitate will deposit on the metal surface. Due to the addition of talc powder (mainly composed of calcium chloride) during the pre flotation de ink process of the waste paper production line, the silicate ions in the sodium silicate stabilizer are easily reacted with cations (mainly Ca2+) in the slurry, resulting in the formation of a difficult to tolerate scale layer at high temperatures.
The use of ultrasonic waves to shock silicon scale in liquid, combined with liquid environments above 100 ° C to 300 ° C, can cause a large amount of silicon scale to fall off, thereby extending the service life of the gear disc.